Table 3.
Effect of different substances on relative activity (%) of purified xylanase from P. glabrum.
| Relative activity (%) | ||
|---|---|---|
| Substance | Concentration | |
| 2 mM | 10 mM | |
| Control | 100 | 100 |
| CuCl2 | 69.7 ± 0.3 | 21.3 ± 0.3 |
| ZnSO4 | 84.4 ± 2.0 | 75.2 ± 1.1 |
| MnSO4 | 178.9 ± 1.6 | 206.7 ± 2.2 |
| BaCl2 | 117.5 ± 1.5 | 124.9 ± 1.7 |
| CaCl2 | 122.0 ± 1.4 | 126.5 ± 1.2 |
| NH4Cl | 102.0 ± 1.55 | 134.3 ± 1.7 |
| NaCl | 99.3 ± 1.3 | 99.2 ± 1.5 |
| SDS | ND | ND |
| PMSF | 92.0 ± 0.9 | 89.3 ± 0.5 |
| MgSO4 | 97.2 ± 1.7 | 97.0 ± 1.2 |
| DTT | 113.4 ± 1.2 | 134.4 ± 2.0 |
| CoCl2 | 117.2 ± 1.6 | 122.2 ± 1.4 |
| HgCl2 | 47.1 ± 0.5 | ND |
| Pb(CH3COO)2 | 68.7 ± 2.0 | 19.1 ± 0.6 |
| EDTA | 89.0 ± 2.0 | 64.8 ± 1.0 |
| β-mercaptoethanol | 124.0 ± 2.0 | 136.0 ± 2.0 |