Fig. 1.

Increased Tregs in the spleens of tumor-bearing mice. a CD4⁺CD25⁺Foxp3⁺ cells in the spleens (n = 4) of naïve and Panc02 tumor-bearing mice were measured by flow cytometry. *p < 0.05. b CD4⁺CD25⁺ T cells were purified from the spleens of naïve or Panc02-bearing mice and co-cultured with OT-I T cells stimulated with OVA_257-264 peptide. Proliferation was measured after 72 h of co-culture. c Depletion of Tregs does not enhance DC immunotherapy. Mice were injected with Panc02 cells. Starting on day 3 and continuing every 3–4 days after, mice received NrIgG or PC-61 antibodies. Mice received DC injections intratumorally on days 3, 7, and 10. Tumor growth was measured