Fig. 5.

Delay of tumor growth and enhanced survival in mice treated with DC and Gem therapy. Mice were injected s.c. with Panc02 tumor cells (n = 8 mice per group). Mice received 120 mg/kg Gem i.p. starting on day 3 and continuing every 3–4 days until day 42. DC vaccines were injected intratumorally on days 3, 7, and 10. Mice received control (PBS), Gem alone (Gem), DC alone (DC), or combination therapy with DC and Gem (DC + Gem). a Tumor size. b Percent survival. c Splenocytes were collected on day 21, and T cells were restimulated for 48 h with media alone (CM), B16 cells, or Panc02 cells. Supernatants were collected, and IFN-γ production was measured by ELISA. d Tumors were collected on day 21. The number of CD3⁺CD8⁺ T cells was calculated per milligram of tumor. e The percentage of CD3⁺CD8⁺ T cells producing IFN-gamma was measured. Data show the mean ± SD (n = 3). *p < 0.05