Table 1. Differential Gene Expression between PT and TH.
A. The of the top ten differentially expressed genes in the PT and TH models, as compared to sham, at the three times points post shock and injury. B. Five additional selected genes of immunological interest that were differentially expressed in the PT and TH models (as compared to sham) at the three different time points after injury and shock.
| A. Top 10 Differentially Expressed Genes | |||||||||||
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| 2 Hours | Day 1 | Day 3 | |||||||||
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| PT | TH | PT | TH | PT | TH | ||||||
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| Genes | Fold Change | Genes | Fold Change | Genes | Fold Change | Genes | Fold Change | Genes | Fold Change | Genes | Fold Change |
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| Down-Regulated | Down-Regulated | Down-Regulated | |||||||||
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| FCER2 | −3.5 | CD55 | −3.2 | MS4A1 | −14 | SS18 | −3.4 | PTGS2 | −15.2 | LY6E | −12.9 |
| HSPH1 | −3.4 | HSPH1* | −3.1 | CD55 | −12.6 | TRIM7 | −3.3 | BACH2 | −11.7 | SBK1 | −12 |
| BCL11A | −3.4 | FCER2 | −3.1 | PTGS2 | −10.1 | RPL41 | −3.2 | LY6E | −11.1 | GNS | −10.2 |
| CD55 | −3.2 | BCL11A | −2.9 | BANK1 | −9.9 | PRPF38B | −3.2 | HIVEP3 | −10.7 | SATB | −10.1 |
| NCR1 | −2.9 | TBX21 | −2.8 | KLRA4 | −9.4 | KLRA4 | −3.1 | SATB1 | −10.4 | BACH2 | −9.6 |
| HPGD | −2.9 | NCR1 | −2.8 | FCER2 | −9.3 | ATR | −3.1 | FCER* | −10.4 | POU2AF1 | −9.1 |
| TARDBP | −2.6 | XCL1 | −2.4 | IVNS1ABP | −9 | RANBP2 | −3 | CCR2 | −10.3 | IL2RG | −9.1 |
| S1PR5 | −2.6 | NRIP2 | −2.2 | P2RY10 | −8.8 | MALAT1 | −3 | III203 | −10.2 | FOXP1 | −8.9 |
| CR2 | −2.6 | EOMES | −2.2 | IGIV1 | −8.8 | HNRPDL | −3 | FILIP1L | −10 | FCER2 | −8.9 |
| XCL1 | −2.5 | CR2 | −2.2 | HLADOB | −8.8 | ZC3H15 | −2.9 | Ifi204 | −9.9 | MLL5 | −8.8 |
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| Up-Regulated | Up-Regulated | Up-Regulated | |||||||||
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| CXCL3 | 227.2 | MMP8 | 30 | SAA1 | 35.1 | RHAG | 17.5 | GSTT1 | 46.7 | GSTT1 | 34.7 |
| CXCL1 | 209.7 | ILR2 | 23 | ARG1 | 16.7 | GSTT1 | 15.9 | RHAG | 27.4 | RHAG | 30.8 |
| IRG1 | 103.8 | MT1E | 17.5 | ASNS | 12.8 | TSPAN8 | 10.8 | ASNS | 17.5 | ASNS | 24.1 |
| NGP | 91.7 | ARG2 | 15.3 | NGP | 11.5 | HFE2* | 8 | CTSF | 15.9 | MGST3 | 17.5 |
| CXCL2 | 57.8 | CXCL3 | 14.6 | GSTT1 | 11.3 | EPB42 | 6.8 | MGST3 | 15.3 | CTSF | 15.4 |
| CD14 | 47.6 | CD14 | 14.5 | HFE2 | 9.4 | OLR1 | 6.4 | NQO1 | 12.1 | NQO1 | 13.6 |
| RND1 | 40.1 | PLSCR1 | 13.6 | PROK2 | 8.9 | ASNS* | 6 | UROD | 12.1 | UROD | 12 |
| IL6 | 39.5 | LCN2 | 13.5 | RHAG | 8.4 | RPH3AL | 5.4 | EPB42 | 11.6 | ATG4A | 11.9 |
| MMP8 | 35.7 | HDC | 13.4 | LIPG | 7.3 | PRNP | 4.6 | ATG4A | 11 | EPB42 | 11.2 |
| CAMP | 35.5 | CRISPLD2 | 12.9 | SPP1 | 6.9 | PTGER3 | 4.3 | NIPA1 | 10.7 | HEMGN | 10.9 |
| B. Selected Genes and their Expression | |||
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| Genes | PT | TH | |
| 2 Hours | TNF | 1.9 | −1.4 |
| CXCL10 | 5.8 | −1.1 | |
| NFKB2 | 3.3 | −1.1 | |
| MAP3K8 | 11.8 | 3.2 | |
| IRAK3 | 6.7 | 1.7 | |
| 1 Day | CD55 | −12.6 | −2.5 |
| CCR6 | −3.7 | −1.8 | |
| CD83 | −5.9 | −2.4 | |
| IL1B | −4.4 | 1 | |
| CD163 | 2.4 | −1 | |
| 3 Days | IL16 | −4.3 | −3.5 |
| CCI3 | −6.4 | −4.2 | |
| FCRIA | −4.6 | −3.1 | |
| BCL21 | 3.1 | 2.3 | |
| H2AB1 | 5.1 | 3.8 | |