Figure 2.
Long-term survey of mice and of engrafted areas after transplantation. Age- and LVFS-matched, female LmnaH222P/H222P mice (n = 26) were randomized into three groups receiving no cell implantation (Sham, n = 8), ESC (ESC, n = 11) or D7LNB1 cells (D7, n = 7, two mice have been killed at 30 days postengraftment). (a) Survival follow-up is represented by cumulative survival curves over a 2-months course. (b) Successive heart sections, representing the whole myocardium from apex to basis, were stained using X-Gal reagent, and means ± SEM of percentages of positive surfaces over the total surface of sections are represented. If any, the surface occupied by ESC was not sufficient for being quantified, and the graph pertains to mice injected with D7LNB1 mice. (c) The calculation of the positive areas delineated the anatomic localization of D7 Mb 30 (n = 2) and 60 (n = 4) days after myocardial injection. Most cell clusters were concentrated at injection sites in the septum or in the left ventricular free wall. ESC, embryonic stem cells; LVFS, left ventricular fractional shortening.