Figure 3.

Remote spread in vitro by transwell assay. (a) Florescence microscopy pictures showing the increased expression of DsRed from vA34R virally infected cells at 24, 36, 48, and 72 hours after infection from the insert wells in both the presence and absence of vaccinia immunoglobulin (VIG), when compared with vvDDr. (b) Flow sorting analysis measuring the DsRED fluorescence in infected cells compared with mock (uninfected) cells in the insert wells at 72 hours shows a threefold and fivefold increase in the percent of DsRed (RFP⁺) fluorescent gated cells with vA34R compared with vvDDr in the absence and presence of VIG, respectively. Due to pooling of all triplicate wells for flow sorting, no means or median values were available.