Figure 3.

KBMA P. aeruginosa is poorly cytotoxic and leads to a mild inflammatory response in vivo. (a) The cytotoxicity of KBMA P. aeruginosa on human moDCs measured by LDH released from dead cells 3 hours after infection at different MOI (1:1; 5:1; 20:1; 100:1) (Mean and SD from five experiments) and (b) the viability analyzed by 7AAD/AnnexinV flow cytometry assay 24 hours after infection with increasing MOI (SD from three different donors). The “-” represents not treated cells. (c) A multiplex immunoassay was used to determine the expression levels of 23 different cytokines in mouse plasma samples that were obtained 24 hours after vaccination (n = 3) with KBMA S54-OVA (triangles) or OSTAB S54-OVA (squares) (5 × 10⁷ bacteria s.c.) versus the phosphate-buffered saline (PBS) control (circles). Only the” cytokines that were significantly different from the control (*P < 0.05) are shown in mice vaccinated with KBMA. The bar represents the mean of the data. KBMA, “killed but metabolically active” MFI, mean fluorescence intensity; MOI, multiplicity of infection.