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. 2013 Mar 26;21(5):1076–1086. doi: 10.1038/mt.2013.41

Figure 5.

Figure 5

The KBMA P. aeruginosa vaccine activates human moDCs and induces antigen-specific CD8+ T-cell responses in vitro. (a) Expression of the maturation markers (CD80, CD86, CD83, and CD40) on the surface of human moDCs infected with live or KBMA strains, at MOI 100:1. (a,b) Not treated lipopolysaccharide (LPS) and CD40L were used as controls. (b) Cytokine secretion by human moDCs following live-strain and KBMA stimulation (n = 5); (c,d) Cross-presentation of flu M1 epitope by DCs submitted to OSTAB or KBMA strains assayed by intracellular IFN-γ labeling within tetramer flu-specific CD8+ T cells from cocultures. T2 and mDC loaded with the epitope were used as controls. (c) One representative experiment, (d) mean of four experiments. (e) Cross-presentation of flu M1 epitope by DCs submitted to OSTAB or KBMA strains assayed by IFN-γ measurement in the supernatants of the cultures (n = 5–8 experiments). HK, heat-killed; KBMA, “killed but metabolically active” MOI, multiplicity of infection.