Fig. 1.

Mutation of wrky8 affects the accumulation of TMV-cg in systemically infected leaves. The third, fourth, and fifth true leaves of 26-d-old wild-type and wrky8 mutants were inoculated with TMV-cg (5 µg/mL solution in 5 mM sodium phosphate, pH 7.5). RNA samples were prepared from systemically infected leaves of six plants inoculated with TMV-cg for 3, 4, 5, 6, and 7 d, respectively, and were probed with a TMV-cg coat protein (CP) cDNA fragment. Ethidium bromide-stained rRNA was used as a loading control. These experiments were repeated three times with similar results. FL, full length.