Fig. 6.

ABI4, ACS6, and ERF104 are direct targets of WRKY8. (A–C) (Upper) Schematic representations of the ABI4, ACS6, and ERF104 promoter regions containing W-box clusters. Only prefect W-boxes (T/CTGACC/T, black bar) are depicted. The diagram indicates the number and relative position of the W-boxes in the respective promoters relative to the ATG start codon. In promoter fragment names, the prefix ”p” indicates promoter. Pink lines indicate the sequences detected by ChIP assays. (Lower) ChIP assays were performed with chromatin prepared from HA-WRKY8 plants infected b TMV-cg at 0, 6, and 7 dpi using an anti-HA antibody (IP) or preimmune serum (no Ab) as a negative control. ChIP results are presented as a percentage of input DNA, SDs were calculated from three technical repeats. One representative experiment is presented. Fig. S3 shows other two ChIP results. The experiments were repeated three times with similar results.