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. 2013 May 6;110(21):8513–8518. doi: 10.1073/pnas.1217988110

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Fig. 5. — Hsp70 is a potent activator of the Hsp104 motor. (A–D) Hsp70 (monomer) or Strep-Tactin (tetramer) was added in n-fold excess over Hsp104 monomer. Averages of three independent measurements ± SD are shown. (A) Hsp70 activates the Hsp104 protein disaggregating activity in a concentration-dependent manner. Recovered enzymatic activities of heat-aggregated β-gal are expressed relative to the complete Hsp104/Hsp70/40 system. (B) Initial rate of β-gal recovery by mixtures of Hsp104 wild-type and Trap mutant at the indicated ratios without and with Hsp70/40. (Inset) An enlarged view of the innate protein remodeling activity of Hsp104. (C) Relative recovery rate of β-gal by Hsp104/Trap and their respective Hsp104_MI variants with Hsp70/40, 10× Hsp70, or 4× Strep-Tactin. The dashed gray line represents the linear decrease expected if the activity of the Hsp104 hexamer is proportional to the number of wild-type subunits present. (D) Relative ATPase activities of Hsp104_MI/Trap_MI without and with Hsp70/40, 10× Hsp70, or 4× Strep-Tactin.