Fig. 2.

Effects of retigabine and ZnPy in the presence of Oxo-M. Perforated patch recording was performed using CHO cells cotransfected with KCNQ2 and human muscarinic type 1 (M1) receptor. KCNQ current was monitored. M1 receptor was activated by 5 μM Oxo-M. (A and B) KCNQ currents in the presence of Oxo-M alone or supplemented with retigabine or ZnPy as indicated. (C) Summary of ZnPy and RTG effects in the conditions as indicated (n = 6). Ratios of current recovery were obtained by normalizing the relative currents at the indicated time point (filled triangle) to the control level (open triangle) (A). (***P < 0.001).