Fig. 5.

Immunofluorescence microscopy images of neurons migrating out of neurospheres, illustrating the asymmetric distribution of nuclear envelope proteins. (A and B) Neurons stained for pericentrin (green), LAP2β (red), and lamin B1 (magenta); (C and D) pericentrin (green) and lamin B2 (red); (E) SUN1 (green) and Nup98 (red); and (F) Nup153 (green) and Nup98 (red). DNA was stained with DAPI (white except Upper area of E and F, where it is blue). In Lmnb1^CS/CS neurons, lamin B2, SUN1, and Nup98 were located largely in the leading end of dumbbell-shaped nuclei (A, C, and E; arrows), but the other end contained nuclear lamina-free chromatin (arrowheads). Small amounts of SUN1 and Nup98 remnants were observed in the trailing end of the dumbbell-shaped nuclei (E). In Lmnb2^CS/CS neurons, nuclear envelope proteins were normally located at the nuclear rim (B, D, F). (Scale bars, 10 µm.)