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. 2013 May 6;110(21):E1923–E1932. doi: 10.1073/pnas.1303916110

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Fig. 6. — Dumbbell-shaped nuclei in Lmnb1^−/− neurons. (A–C) Immunofluorescence microscopy images of Lmnb1^+/+ and Lmnb1^−/− neurons migrating from neurospheres, showing the asymmetric distribution of lamin B2 and nuclear pore proteins Nup98 and Nup153 in Lmnb1^−/− neurons. (A) Lmnb1^+/+ and Lmnb1^−/− neurons stained with antibodies against pericentrin (green), LAP2β (red), and lamin B1 (magenta); (B) pericentrin (green) and lamin B2 (red); (C) Nup98 (red) and Nup153 (green). DNA was visualized with DAPI (white except Upper area of C, where it is blue). (D) Frequency of dumbbell-shaped nuclei in WT and mutant neurons. From left to right, Lmnb1^+/+ (wild-type), n = 4; Lmnb1^CS/CS (B1CS), n = 5; Lmnb2^+/+ (WT), n = 1; Lmnb2^CS/CS (B2CS), n = 1; Lmnb1^+/+ (WT), n = 4; Lmnb1^+/− (HET), n = 3; Lmnb1^−/− (B1KO), n = 3. For each cell line, >180 cells were counted by two independent observers in a blinded fashion. Values represent mean ± SD.