Fig. 4.

SGK1 mediates and maintains CORT effects on GR nuclear translocation. (A) Cotreatment with CORT (100 µM) and GSK650394 (100 nM) for 12 h. (B) GSK650394 counteracted the CORT-induced increase in nuclear GR (n = 7). (C) The CORT-induced decrease in cytoplasmic GR was not counteracted by GSK650394 (n = 7). (D) CORT treatment for the first 3 h and GSK650394 treatment during the subsequent 9 h in the absence of any CORT. (E) CORT (for only 3 h) was sufficient to induce GR nuclear translocation after a total incubation of 12 h. GSK650394 (during the 9-h period after cortisol) abolished the CORT-induced increase in nuclear GR (n = 5). (F) CORT (for only 3 h) decreased cytoplasmic GR. This effect was not counteracted by subsequent GSK650394 treatment (n = 5). Data are mean ± SEM *P < 0.05, **P < 0.01.