Fig. 5.

SGK1 effects on proliferation occur upstream and downstream of the GR. (A) CORT (100 µM) for only the first 3 h of a 72-h incubation period was sufficient to reduce the number of BrdU-positive cells (first column). Treatment with GSK650394 (100 nM) during the 69-h period after the initial 3-h CORT stimulus abolished the CORT-induced reduction in proliferation (second column), whereas the GR antagonist RU486 (50 nM) partially counteracted the effect of CORT (third column). n = 10. Data are mean ± SEM *P < 0.05, **P < 0.01; ***P < 0.001. (B) Diagram depicting the proposed model of SGK1-dependent regulation of neurogenesis and GR function.