Fig 1.

Details of the RPA amplicon for VARV. All nucleotides in the alignment matching in the VARV sequence are presented as dots. Primer sequences are presented as full sequences. Gaps are presented as hyphens. VARV RPA FP is presented in sense, and VARV RPA P and RP are presented as reverse complement sequences. Gray fields: VARF RPA FP, degenerated IUB code positions; VARV RPA P, the TTT triplet used for the attachment of BTF (Table 2); VARF RPA RP, nucleotide at position 3 of the 3′ end mismatching all other orthopoxviruses. Sequences: cowpox virus, AY902252; camelpox virus, AF438165; monkeypox virus, AF380138; vaccinia virus, M35027; variola virus, X69198.