Table 1.
Details of RPA amplicon design
| Infectious agent | Target gene | Reference sequence, position(s) of RPA amplicon | RPA amplicon length (nt) | Sequences used in the design |
|---|---|---|---|---|
| Y. pestis | pla | AF053945, 7267–7420 | 153 | AF528089, AF053945, AL1009969, NC_003132, NC_004837, AE017046, NC_005816, CP001592, CP001596, NC_014027 |
| B. anthracis | pagA | CP001216, 144299–144424 | 125 | AF306778-83, AE011190, NC_003980, AE017336, CP001216, NC_012579, CP001599, NC_012656 |
| B. anthracis | capC | AF188935, 55735- 55885 | 150 | AF188935, AE01191, NC_003981, AE017335, NC_007323, CP001214, NC0125771, CP001597, NC_012655 |
| Variola virus | HA | X69168, 151606- 151732 | 126 | X69168, Y16780, DQ441416, DQ441418-48, DQ437500, DQ437581-91 |
| Vaccinia virus | HA | DQ121394, 165441- 165584 | 143 | M35027, U94848, AY243312, AY313847-48, AY603355, NC_006998, DQ121394 |
| Ebola virus | NP | AY142960, 1779–1943 | 164 | Jo44337, L11365, AF086833, AY142960, AF499101, AF272001, EU22440, AY354458, Y09358, AY054908, AY058895, EU051640-50 |
| Sudan virus | NP | AF173836, 1783- 1868 | 130 | AF173836, AY729654, NC_006432, EU338380 |
| Marburg virus | NP | FJ750959, 1121–1256 | 135 | Z12132, Z29337, NC_0016081, DQ217792, AY430365-66 |
| Marburg virus | NP | FJ750953, 1121–1260 | 107 | X68495, M72714, DQ447649-61, AY358025, FJ750953-59 |
| Sigma virus | G | X06171, 84–960 | 119 | X00171 |