Table 3.
Sensitivity of RPAs
| Infectious agent (target gene) | Real-time PCR sensitivity (three runs) (reference) | RPA sensitivity (eight runs) (reference) | RPA probit 95% sensitivity (eight runs) | RPA threshold time to sensitivity limit (min) | Sensitivity LAMP (reference) |
|---|---|---|---|---|---|
| DNA assays | |||||
| B. anthracis (pagA) | 101 to 102 (21) | 101 to 102 | 16 | 8 | 103 (27) |
| B. anthracis (capC) | 102 to 103 (21) | 102 to 103 | 778 | 7 | 103 (27) |
| F. tularensis (tul-4) | 102a (28) | 101 to 102 (35a) | 19 | 10 | NDc |
| Y. pestis (pla) | 2a (29) | 101 to 102 | 16 | 8 | ND |
| Variola virus | 101 to 102 (30) | 101 to 102 | 16 | 10 | 102b (31) |
| RNA assays | |||||
| Rift Valley fever virus (N) | 102 | 101 to 102 (35b) | 19 | 7 | 102 (32, 33) |
| Ebola virus (NP) | 102 | 101 to 102 | 21 | 7 | 101 (34) |
| Sudan virus (NP) | 101 | 101 to 102 | 17 | 8 | ND |
| Marburg virus (NP) | 101 | 101 to 102 | 21 | 8 | 102 (35) |
| Sigma virus (G) | 101 | 101 to 102 | 16 | 4 | ND |
a
Calculated from the fg value given in the source reference publication.
b
As determined from the monkeypox LAMP assay.
c
ND, not determined.