. 2013 Apr;51(4):1124–1129. doi: 10.1128/JCM.03113-12

Table 2.

Target genes, amplicon sizes, labels, and sequences of primers and probes used in the Luminex assay for detection of influenza viruses (FLULUM)

Target gene	Amplicon size (bp)	Function^a	Sequence (5′-3′)^b
FluA matrix	83	Forward	`TGGARTGGCTAAAGACAAGAC`
		Reverse^*	`CACTGGGCACGGTGAGCGTGAACAC`
		Probe^†	`CACCTCTGACTAAGGGRATTT`
FluA hemagglutinin H3	98	Forward	`CTGGAGAACCAACATACAATTG`
		Reverse^*	`GCCCATATCCTCAGCATTTTCCCTCAGTTG`
		Probe^†	`CTGACTCAGAAATGAACAAACTGTT`
FluA hemagglutinin H1	122	Forward	`GAAATATTCCCCAAAGARAGCT`
		Reverse^*	`ACCATTCTTCCCCGTCAGCCATAGCAAA`
		Probe^†	`TGGCCCAACCACACCGTAACCG`
FluA hemagglutinin 2009 H1	115	Forward	`GGTGCTATAAACACCAGCCT`
		Reverse^*	`GGGAYATTCCTCAATCCTGTGGCCAGTCTC`
		Probe^†	`CCCATTTCAGAATATACATCCGAT`
FluB matrix	90	Forward	`GAAGATGGAGAAGGCAAAG`
		Reverse^*	`TCCATTCCAAGGCAGAGTCTAGGTCA`
		Probe^†	`GCAGAAAAATTACACTGTTGGTT`
Jellyfish (EXO)	84	Forward^*	`CAAATTGAACGGTCAATTGGAAGTGG`
		Reverse	`CCTAAGACAAGTGTGTTTATGG`
		Probe^†	`CGTTTGCAATAGCTGTTCTTC`

*, Primer labeled with 5′ biotin; †, probe modified with 5′ amino-C12 linker.

Degenerate bases: R, A or G.