Table 1.

T. vaginalis PCR primers and their sensitivities^a

Gene targetb	Primer	Primer sequence	Size (bp)	Sensitivityc
				PCR	Nested PCR
KENGOE (L23861)	TVK3F	5′-ATT GTC GAA CAT TGG TCT TAC CCT C-3′	262	1	0.01
	TVK7R	5′-TCT GTG CCG TCT TCA AGT ATG C-3′
	TVC1F	5′-TCA GTT CGC AAA GGC AGT CCT-3′	213	1
	TVC2R	5′-GTA CTT ACG CTT GGA GAG GAC ATG A-3′
MURESU (X83109)	TVC3F	5′-GAT GCC ATG AAC GGA AAT GTT-3′	299	1	0.01
	TVC4R	5′-TCT GGA GCA TAT TGG ATC CG-3′
	TVC11F	5′-CGA ATG GRA TAA CGA ATG CGA C-3′	237	0.1
	TVC12R	5′-CAA CCT TTC TTG TCA GAC AAC TTG-3′
PACES (M86482)	TVC5F	5′-AAT TCC CGG ATA ATT GAA ACG GA-3′	190	0.1	0.01
	TVC6R	5′-GAT GTT GGG GAT GTT TTG TAT TCT G-3′
	TVC7F	5′-GAT AAA GAA AAT GTG TTT AAG TTG ATG GA-3′	148	1
	TVC8R	5′-TTG TAT TCT GAC ACT GGT TCC AAT TT-3′
PACES (M86482)	TVOP1F	5′-GTG AAA ATC TCA TTG GGG TAT TAA CTT-3′	580	1	0.01
	TVOP2R	5′-GTT TTA TTT ATC ACT GGA AAA TAA CGC TT-3′
	TVC9F	5′-AGA ATA CAA AAC ATC CCC AAC ATC TT-3′	358	1
	TVC10R	5′-CCC ATT CTT TTA GAC CCT TCA GAT T-3′

^aFor each gene target, the top 2 primers are for 1st PCR and the bottom 2 primers for 2nd PCR.

^bThe gene targets are labeled with the name of the first author in the published sequence (GenBank accession number).

^cT. vaginalis organisms per PCR.