Table 2.

Primers used for nested amplification (TF1/TR5 [1st PCR] and TF2/TR2 [2nd PCR]) and sequencing (TF3, TR1, TR3, and TR4) of the Trichomonas 5.8S rRNA gene and the flanking internal transcribed spacer region ITS1

Primer	Primer sequence
TF1	5′-TCC TAC CGA TTG GAT GAC TCG-3′
TF2	5′-GGA AGG AGA AGT CGT AAC AAG-3′
TF3	5′-GTA GGT GAA CCT GCC GTT GGA T-3′
TR1	5′-TGA GGA GCC AAG ACA TCC ATT G-3′
TR2	5′-ATG CAA CGT TCT TCA TCG TG-3′
TR3	5′-GCG CAA TTT GCA TTC AAA GAT-3′
TR4	5′-GAG ATG CTT CAG TTC AGC GGG T-3′
TR5	5′-CTT TTC CTC CGC TTA TTG AGA TG-3′