Table 2.
Sequence | Count | % | No. of differences | Predicted % bound at annealing temp |
---|---|---|---|---|
.................... | 2,121 | 97.5 | 0 | 100 |
.....T.............. | 14 | 0.64 | 1 | 94.4 |
..............G..... | 6 | 028 | 1 | 81.4 |
.....R.............. | 6 | 0.28 | 1 | 87.9b |
.....W.............. | 5 | 0.23 | 1 | 94.4c |
..............C..... | 5 | 0.23 | 1 | 94.5 |
.....G.............. | 4 | 0.18 | 1 | 87.9 |
Variability is represented relative to the reference sequence at each site; a period is shown where the test sequence matched the reference exactly. The top line represents the most commonly found variant. Each individual sequence variant that was represented at >0.1% frequency in the data set is shown along with its prevalence, the number of differences compared to the target sequence, and the predicted binding efficiency.
Percentage shown is for the G variant.
Percentage shown is for the T variant.