Table 2.

Survey of technologists who routinely used CHROMagar Orientation (CO) medium^a

Question	No. of responses (% of total)
	2008 data		2012 data
	Yes	No	Yes	No
1. Do you routinely (>80% of the time) have sufficient growth on CO medium for susceptibility testing on day 1 (i.e., subculture not required before testing) for
E. coli	18/18 (100%)	0	34/34 (100%)	0
Enterococcus	11/18 (61%)	7/18 (39%)	16/34 (47%)	18/34 (53%)
Klebsiella Enterobacter Serratia group	18/18 (100%)	0	34/34 (100%)	0
Other Gram-negative bacilli	18/18 (100%)	0	34/34 (100%)	0
Other Gram-positive cocci	16/18 (89%)	2/18 (11%)	18/34 (53%)	16/18 (47%)
2. Do you encounter difficulties with any spot/rapid tests that are performed from CO medium?	1/18 (6%)	17/18 (94%)	5/34 (15%)	29/34 (85%)
3. Does CO medium produce consistent color for E. coli and Enterococcus to allow you to report the organism without additional tests?	17/18 (94%)	1/18 (6%)	30/34 (88%)	4/34 (12%)
4. Do you feel CO medium aids in distinguishing mixed cultures?	17/18 (94%)	1/18 (6%)	32/34 (94%)	2/34 (6%)
5. Do you find colors on CO medium are easy to distinguish for the majority of isolates?	17/18 (94%)	1/18 (6%)	34/34 (100%)	0
6. Do you feel CO medium has improved efficiency on the bench?	18/18 (100%)	0	33/34 (97%)	1/34 (3%)
7. Do you feel confident in reporting E. coli and Enterococcus without additional identification tests?	18/18 (100%)	0	32/34 (94%)	2/34 (6%)
8. What percentage of the time do you need to subculture organisms from CO medium before starting workup?
≤10%	17/18 (94%)		31/34 (91%)
11–50%	1/18 (6%)		3/34 (9%)
>50%	0		0
9. To help identify organisms that will be “listed” in a final report, what percentage of the time do you need to do a Gram stain?
≤10%	6/18 (34%)		7/34 (21%)
11–50%	8/18 (44%)		15/34 (44%)
>50%	4/18 (22%)		12/34 (35%)

^aThe survey was of technologists who routinely used CHROMagar Orientation (CO) medium at the urine culture bench 1 month after and 4 years after it was implemented as the primary medium in the clinical microbiology laboratories.