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. Author manuscript; available in PMC: 2014 Feb 11.
Published in final edited form as: ACS Comb Sci. 2013 Jan 25;15(2):77–81. doi: 10.1021/co300135r

Figure 2.

Figure 2

Model DARPin selections using our streamlined mRNA display protocol. Four binary libraries containing mRNAs encoding H10-2-G3 (H: a Her2 binder) and Off7 (O: a MBP binder) at different molar ratios (1:10, 1:100, 1:1,000, and 1:10,000) were subjected to two to three rounds of selections against immobilized Her2. The original library (Ori.) and selective enrichment of H10-2-G3 in the RT-PCR product after each round of mRNA display (R1, R2, and R3) is visualized by agarose gel electrophoresis. Each selection round took two days in the laboratory.