Table 1.
Top 20 motifs from motif search
| 5′ UTR motifs selected for fast translation |
Frequency | 5′ UTR motifs (18b prior to AUG) in E. coli |
Frequency |
|---|---|---|---|
| GGAGG | 0.242 | AGGAG | 0.156 |
| AGGAG | 0.161 | AAGGA | 0.155 |
| GAGGA | 0.119 | GGAGA | 0.116 |
| GAGGU | 0.117 | CAGGA | 0.114 |
| GAGGG | 0.099 | AGGAA | 0.114 |
| GGGAG | 0.078 | GAGGA | 0.090 |
| AAGGA | 0.077 | AAAAA | 0.085 |
| AGGUA | 0.075 | AAAGG | 0.084 |
| UAGGA | 0.061 | GGAGU | 0.084 |
| GGUAU | 0.056 | GAGAA | 0.079 |
| GGUGG | 0.055 | ACAGG | 0.075 |
| UGAGG | 0.054 | GGAGG | 0.075 |
| GUGGG | 0.053 | UGGAG | 0.073 |
| UAAGG | 0.051 | UAAGG | 0.071 |
| UUAUU | 0.050 | GAGGU | 0.069 |
| GGGUG | 0.050 | GGAAA | 0.066 |
| AGGGA | 0.049 | AGAGG | 0.064 |
| UGGAG | 0.049 | GAAAA | 0.063 |
| UAUUU | 0.048 | AGGAU | 0.063 |
| UUAGG | 0.047 | AAAAG | 0.063 |
5′ UTR motifs selected for ability to facilitate translation in an E. coli-based translation system exhibit high similarity to the same region (18 bases prior to AUG) in E. coli. The top 20 five-base motifs from each population are shown. Seven particular motifs (bold and underlined) were present in both sets. Similar results were obtained using other motif lengths. 5′ UTR, 5′ untranslated region.