Fig 6.

Surface mutations on Asf1's conserved core display various degrees of silencing. (A and B) The Asf1-H3/H4 complex is shown docked with the B-domain peptide of HIRA (blue). Asf1 colored according to sequence conservation, with absolutely conserved residues (red), highly similar residues (pink), and the least-conserved residues (white) mapped onto the surface. (C) Wild-type strain ROY1172 (ASF1 CAC1) carrying an empty vector marked with TRP1 or ROY1169 (asf1Δ cac1Δ) carrying plasmid-borne copies of either WT or mutated Asf1 (strains BKD094, -095, -103, -104, -106, -107, -109, and -110) were serially diluted 10-fold onto the indicated media.