Table 3.
MS confirmation that Asf1 T270, not T265, is phosphorylated in vivo
| Straina | Peptideb | Scorec | Expect (P) value |
|---|---|---|---|
| Asf1 | KIEGGSTDIESTPK | 41.5 | <0.05 |
| KIEGGSTDIEStPK | 36.0 | ||
| KIEGGSTDIEsTPK | 34.2 | ||
| IEGGStDIESTPK | 20.9 | ||
| T265A | IEGGADIESTPK | 36.9 | <0.05 |
| IEGGADIEStPK | 23.2 | ||
| T270A | KIEGGSTDIESAPK | 45.3 | <0.05 |
| IEGGSTDIESAPK | 33.7 | ||
| T265A | KIEGGSADIESAPK | 43.0 | <0.05 |
| T270A | IEGGSADIESAPK | 34.5 |
a
Tandem MS performed on Asf1 immunoprecipitated from JKT200 strains possessing pAsf1-Myc or mutagenized pAsf1-Myc showed that only Asf1 from strains in which Asf1-T270A was present prevented the identification of phosphorylated peptides.
b
Residues in lowercase were identified as phosphorylated.
c
The scores are discriminate scores, a statistical measure of confidence in the identification of individual peptides generated by the Mascot or Protein Prospector analysis program.