Figure 3. EvTI partial primary sequence alignment with sequences of known legume seeds Kunitz-type trypsin inhibitors: E. velutina (EvTI); E. caffra (TSI) (gi 157833954); E. variegata (ETIb) (gi 262753); Psophocarpus tetragonolobus (WBTI) (gi 86450987); Bauhinia variegata (BvcTI) (gi 15082208); Bauhinia ungulate (gi 32363179); Prosopis juliflora (PjTKI) (gi 243387); Adenanthera pavonina (ApTKI) (gi 225058).

Sequences deposited in the database of NCBI (National Center for Biotechnology Information) were compared using the BLASTp. The alignment was performed using Bio Edit software v 7.0.9.0 through the Clustal W multiple alignment tool.