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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Anal Chem. 2012 Dec 12;85(2):749–768. doi: 10.1021/ac3033257

Figure 2. Specificity of interactions in IP experiments.

Figure 2

A) Sources of non-specific interactions include associations with resin, and antibody, as well as isolated proteins. B) A balance between stringency of lysis buffer and incubation period with the antibody can aid in isolating specific interactions, while reducing the presence of non-specific interactions.