Figure 2.

Trip12 protein expression in adult mouse tissues and interaction between endogenous Sox6 and Trip12 proteins. (A) Western blot analysis was performed to determine Trip12 protein expression in adult mouse tissues. Each lane contained 30 μg of protein except for the C12C12 samples, which contained 15 μg of protein per lane. Relevant protein size markers (kDa) are indicated to the left. β-actin was used as a loading control. (B) Co-IP of endogenous Sox6 and Trip12 proteins using nuclear fractions of differentiating C2C12 cells. Input lane contained 5% (15 μg) of pre-cleared nuclear protein. Antibodies used for pull down are listed under IP, and antibodies used for Western blot (WB) are indicated below each panel. GST antibody was used as a negative control.