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The cells were immunostained with CGβ antiserum (green) and monoclonal antibody against FSHβ subunit (green). Note unique ER/perinuclear staining pattern for LHβ (A, arrow) vs. dispersed cytoplasmic puncta for FSHβ subunit (B, arrowhead). The n indicates the nucleus (red). The micrographs shown are representative of four to eight experiments and are at the X100 and X150 magnification. NRS (C), normal rabbit serum.
