Skip to main content
. 2013 May 29;8(5):e65259. doi: 10.1371/journal.pone.0065259

Figure 1. Design of 15-RVD GoldyTALENs.

Figure 1

The design parameters of active 15-RVD GoldyTALEN pairs used in this study. Each TALEN arm consists of a DNA binding domain with 15-RVDs (14.5 TALE repeats), corresponding to 15-nucleotides DNA binding sequence proceeded by a 5′ T nucleotides and a 13 to 20 bp spacer in between 2 arms containing a restriction recognition sequence to assay activity. *Initial design parameter was spacer length between 11–20 bp and only the two inactive pairs, NPM1B P1 and P2 have spacers shorter than 13 bp.