Figure 5. Deletion of large genomic fragments with two pairs of TALEN.

(A) Schematic diagram showing the strategy of introducing large genomic deletions at flt3 and jak2a loci using two TALEN pairs. Green and purple arrows represent primer pairs used for PCR screening of corresponding large deletion. WT: wild-type; T: TALEN pairs injected. (B) Sequences of germline-transmitted large deletions in F1 embryos. Sequence of a single mutant F1 embryo from each founder is shown. Underlined are TALEN binding sites. Sequences in blue represent insertions.