Table 1. Correlation of spacer lengths and somatic activities of 15-RVD GoldyTALENs.
TALEN Pair* | Spacer Length (bp) | Somatic Activities (% chromosomal conversion) |
IDH1 P1 | 16 | 86±3%* |
FLT3 P1 | 16 | 84±5%* |
FLT3 P2 | 15 | 83±4%* |
NPM1B P1 LN | 15 | 83±7%* |
JAK2A P1 | 19 | 78±9% |
NPM1B P1 RN | 15 | 71±8% |
FLT3 P3 | 14 | 67±6% |
GFP(GM2) P1 | 19 | 52±4% |
JAK2A P3 | 18 | 45±3% |
NPM1A P1 | 13 | 38±4% |
JAK2A P4 | 15 | 36±3% |
NPM1A P2 | 18 | 35±6% |
JAK2A P5 | 19 | 28±3% |
JAK2A P2 | 16 | 24±3% |
IDH1 P1 RM† | 17 | 15±4% |
JAK2A P1 LM† | 18 | 9±3% |
NPM1B P2 | 11 | 3±1% |
NPM1B P1 | 12 | 4±1% |
shown in decreasing order of activity.
modified without the 5′ T nucleotide at one of the TALEN arms.