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. 2013 May 2;13:221. doi: 10.1186/1471-2407-13-221

Figure 1.

Figure 1

Established colon cancer cell lines grow as disc (flat) or round (spheroid) colonies in 3D. A) Phase images of HCT-116 cells grown in 3-D matrigel, with EGF (E), Noggin (N), Wnt3a (W), and R-Spondin1 (R) (RNEW) media. Images were captured on day 1, 2, 3, 4, and 6, and show colonies that grew as round spheres. B) Phase images of HCT-116 cells grown in 3-D matrigel as in (A). Images show colonies that grew as flat discs. C) Summary of colony phenotypes of colon cancer cell lines grown in 3-D matrigel with RNEW media. Top row shows percentage of disc colonies, with SEM in parenthesis, n>3. Subsequent rows depict mutational status of genes known to drive in colon cancer development RCGDB (Roche Cancer Genome Database [16]).