Figure 6.

Growth factors did not induce invasive characteristics in 2-D cultures. A) Confocal images of HCT-116 cells grown in 2-D with E or RNEW media for 6 days. a, c, and e: cells grown in E media; b, d, and f: cells grown in RNEW media; a-b: E-cadherin merged with DNA; c-d: β-catenin merged with DNA, and e-g: actin merged with DNA. B) Quantitation of luciferase activity of the TOPFLASH-reporter. TOPFLASH-luciferase and β-galactosidase reporters were co-transiently transfected into HCT-116 cells. 24 hours later, cells were treated with the indicated growth factors for an additional 24 hours and luciferase and β-galactosidase were measured. Values are normalized to β-galactosidase activity. Results expressed as the mean percent of normalized luciferase activity +/− SEM, n=3.