Figure 7.

Effects of cinnamic acid on microtubules organization of HT-144 cells. Images obtained by Laser Scanning Confocal Microscopy of anti-tubulin immunofluorescence (blue) preparations: A) interphasic HT-144 control cells; B) mitotic HT-144 control cell; C,D) HT-144 cells treated with 3.2 mM cinnamic acid. DNA was counterstained with propidium iodide (red). We can observe cells with a microtubule network that was very finely departed from the centrosome region near the nucleus (up left) and a normal mitosis (up right). On the other hand, we found cells with microtubule disorganization and tubulin bunches near the nuclei.