Figure 2. Specificity and broad dynamic range of peptide arrays.

(a) Images displaying reactive peptide features from an array probed with HA-tag antibody (eBioscience 14-6756-81). Amino acid sequence of peptide targets is displayed in parentheses. Residues corresponding to HA-tag sequence (NH₂-YPYDVPDYA-COOH) are underlined in seasonal H3N2 peptide sequences. (b) Graph displaying MFI of array peptide features depicted in a in a titration experiment using HA-tag antibody ranging in concentration from 2000 ng mL⁻¹ to 0 ng mL⁻¹. Error bars in b reflect mean ± SEM of triplicate array feature MFI for each titration point.