Figure 1. Gene and protein expression in plin+/+, +/− and −/− mice adipose tissues.

(A) mice WATs. (B) Total RNA was extracted and reverse transcribed from tissues. Quantitative RT-PCR was performed using Taq-Man Gene Expression Assays. S17 rRNA was used as an internal control to normalize the mRNA levels. Data are means ±SD of 5 or 6 mice. The relative mRNA level of each gene in plin+/+ mice is taken as 1.0. *p<0.05 and **p<0.01 using unpaired Student’s t test. (C) Proteins were extracted from tissues and immunoblots were performed. P and N denote the precursor and nuclear form of SREBPs, respectively.