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. 2013 May 29;8(5):e64605. doi: 10.1371/journal.pone.0064605

Figure 3. Enforced expression of plin in plin−/− MEFs promotes LD generation and SREBP-1 activation.

Figure 3

(A) Pictures of Oil Red O staining of MEFs infected with the mock or 3xFlag-plin virus. Staining was performed on days 0 and 6. (B) Quantitative RT-PCR analyses showing the gene expression patterns in mock or plin expressing MEFs (n = 3). S17 rRNA was used as an internal control to normalize the mRNA level of each gene. Data are means ±SD; the levels in mock cells on day 0 are taken as 1.0. *p<0.05 and **p<0.01 versus mock. (C) Immunoblots showing the levels of proteins in mock or plin expressing MEFs during adipogenesis. P and N denote the precursor and nuclear form of SREBP-1, respectively.