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. Author manuscript; available in PMC: 2013 May 29.

Published in final edited form as: Biochemistry. 2011 Oct 27;50(46):10114–10125. doi: 10.1021/bi201092q

Table 2. Enzyme kinetics parameters for PTP10D and the F76L mutant determined using different substrates.

PTP10D
	V_max (μmole/min/mg)	K_m (μM)	K_cat (sec⁻¹)	K_cat / K_m (sec⁻¹ M⁻¹) × 10³
pNPP	13.74 ± 1.40	5312.80 ± 58.42	8.24 ± 0.89	1.55 ± 0.16
GP1	10.45 ± 0.48	138.05 ± 6.43	6.65 ± 0.32	48.18 ± 2.39
GP2	6.24 ± 0.55	508.11 ± 45.21	3.92 ± 0.34	7.71 ± 0.67
GP3	10.33 ± 0.41	129.67 ± 5.07	6.47 ± 0.26	50.15 ± 2.11
GP4	6.82 ± 0.24	69.88 ± 2.24	4.27 ± 0.15	61.11 ± 2.14
PTP10D F76L mutant
	V_max (μmole/min/mg)	K_m (μM)	K_cat (sec⁻¹)	K_cat / K_m (sec⁻¹ M⁻¹) × 10³
pNPP	8.01 ± 0.84	24891.9 ± 387.09	4.80 ± 0.50	0.19 ± 0.02
GP1	3.33 ± 0.39	1081.24 ± 133.17	2.00 ± 0.24	1.85 ± 0.22
GP2	0.83 ± 0.11	2564.83 ± 355.26	0.52 ± 0.07	0.21 ± 0.03
GP3	3.54 ± 0.46	1009.29 ± 137.87	2.12 ± 0.27	2.11 ± 0.27
GP4	1.49 ± 0.13	542.95 ± 48.67	0.89 ± 0.08	1.64 ± 0.15