. Author manuscript; available in PMC: 2013 May 29.

Published in final edited form as: Biochemistry. 2011 Oct 27;50(46):10114–10125. doi: 10.1021/bi201092q

Table 4. Kinetic parameters, rate constants and the half life of PNC binding obtained for PTP10D and mutant proteins.

PTP10D	t₀ (sec)	α (A.U.)	β (A.U./sec) × 10⁻²	t_1/2 (sec)
Wild Type	50.0	0.88 ± 0.01	35.97 ± 0.04	62.89 ± 0.04
F76L	160.0	0.87 ± 0.03	4.57 ± 0.01	197.22 ± 0.25
C242S	35.0	0.90 ±0.01	12.51 ± 0.07	52.14 ± 0.08
D210A	30.0	0.87 ± 0.02	42.01 ± 0.05	37.73 ± 0.05
C242S/Q286A	40.0	1.04 ± 0.02	2.49 ± 0.03	111.07 ± 0.14
D210A/Q286A	16.0	0.85 ± 0.02	44.24 ± 0.04	22.9 ± 0.05