Figure 3.

Heat map representation of miRNAs modulated in human DL06 myeloma line by CD38-mediated signals. Total RNA was isolated from DL06 line treated with anti-CD38 (clone IB4) mAb for 0, 2, 6 and 24 h at 4°C. An irrelevant isotype-matched anti-TCRvβ3 mAb was used as control for the same interval times. Two biological replicates for 0, 24 h and control samples were hybridized. miRNA expression was investigated using the Agilent Human miRNA microarray (G4470B, Agilent Technologies), as described (69). By GeneSpring GX 12 software (Agilent Technologies), data transformation was applied to set all the negative raw values at 1.0, followed by a quantile normalization and a log₂ transformation. miRNA expression was referred to time 0 h of each biological series. Filters on gene expression were used to keep only the miRNAs that were detected in at least one sample. miRNAs significantly modulated in the DL06 model after 24-h treatment with agonistic anti-CD38 mAb were identified using unpaired t test (corrected p value <0.05). Treatment was included in cluster analysis. The expression values of the genes represented on the heat map correspond to the values normalized on time 0 sample of the same biological series. Red represents upregulated expression and green downregulated expression.