Figure 4.

Givinostat reduces fibrosis in mdx muscles. (A) Representative images of Masson trichrome staining of TA transverse sections of CTR-, TSA- or givinostat-treated mdx mice (givinostat 1 mg/kg/d = ITF1; givinostat 5 mg/kg/d = ITF5; givinostat 10 mg/kg/kg = ITF10) at the end of the treatment. Original magnification, 10×. (B) Graph representing the fibrotic index (quantification of collagen deposition) of muscles shown in (A) measured as blue area (reported as pixel²) per field. Data are presented as the average ± SEM; n = 6. ***p < 0.001.