Fig 2.

Isoflurane altered GFAP expression both during and 48 h after treatment. GFAP, a marker of astrocytes, is strongly expressed in control astrocyte cultures. (a) Isoflurane led to a decrease in GFAP expression both during treatment and (b) 48 h later. (c) GFAP image 48 h after exposure from control plate. (d) GFAP image 48 h after anaesthesia from isoflurane-treated plate. Data expressed as a percentage of control values [mean (sd), n=20 wells per treatment condition]; GFAP Alexa Fluor® 488 (green), Hoechst (blue). Isoflurane also led to a decrease in GFAP gene expression as measured by RT-PCR at the end of treatment (e) but this had resolved by 48 h later (f) (n=3, per treatment condition), *P<0.05, **P<0.01, ***P<0.001.