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. 2013 Feb 14;133(6):1533–1540. doi: 10.1038/jid.2013.4

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Copyright © 2013 The Society for Investigative Dermatology, Inc

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Histopathology and TUNEL-positive cells in cutaneous leishmaniasis (CL) lesions. (a, b) Necrosis scores 1 and 2 showed by arrowhead and inset. (c) Analysis between intensity of necrosis and percentage of CD8⁺Granzyme B⁺ T cells. DNA damage in biopsies with (d) higher and (e) lower percentages of TUNEL-positive cells, which are indicated by black arrows. (f) Correlation analysis between the percentage of CD8⁺Granzyme B⁺ T cells in the tissue and the percentage of TUNEL⁺ cells. (g) Analysis between the intensity of necrosis and the number of TUNEL⁺ cells. Bar=20 μm. *P<0.05. Statistical comparisons were done using the Mann–Whitney U-test and the Spearman's (r²) rank test. Pictures represented a magnification of × 200, insets × 1,000, and TUNEL⁺ cells × 400.