Table 2.
Chromosomal region (gene) | Marker ID | Nucleotide sequence of primers (5′ → 3′) |
---|---|---|
3p14.2 (FHIT) | D3S1234 | Fa CCTGTGAGACAAAGCAAGAC—F |
Ra GACATTAGGCACAGGGCTAA | ||
D3S1300 | F AGCTCACATTCTAGTCAGCCT—F | |
R GCCAATTCCCCAGATG | ||
3p21.3 (RASSF1A) | D3S3615 | F TGGAAAGGTAAGCACAAGC—N |
R TCCTCCCAGGAAGCAC | ||
3p22.2 (MLH1) | D3S1611 | F CCCCAAGGCTGCACTT—V |
R AGCTGAGACTACAGGCATTTG | ||
3p24.2 (RARβ) | D3S1583 | F AGCTTGTAAATAGGTCCTAACAGAG—N |
R TGGTTTAATAGGCACCGTTT | ||
3p25.3 (VHL) | D3S1317 | F TACAAGTTCAGTGGAGAACC—F |
R CCTCCAGGCCATACACAGTCA | ||
D3S3611 | F GCTACCTCTGCTGAGCAT—V | |
R TAGCAAGACTGTTGGGG |
F, 6-FAM; P, PET; N, NED; V, VIC
a F forward (sense), R reverse (antisense)