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. Author manuscript; available in PMC: 2013 May 30.
Published in final edited form as: Lasers Surg Med. 2012 Jan 3;44(1):60–68. doi: 10.1002/lsm.21160

Fig. 2.

Fig. 2

HPPH-PDT does not affect classical MHC class I molecule expression. A: Colo205 cells treated with HPPH-PDT at 0.3 J/cm2 were harvested immediately following, or 1, 3, 6, 12, and 24 hours post-treatment. cDNA generated from each of the time points was used in Q-PCR reactions using primers specific to β2-microglobulin (upper panel) or MHC class I heavy chain (lower panel). Induction was normalized to both GAPDH and β-actin. B: Total protein levels for the indicated proteins were examined 1, 6, 12, and 24 hours post-HPPH-PDT at 0.3 J/cm2. C: The surface expression of classical MHC class I molecules was determined as a function of fluence 24 hours post-HPPH-PDT. D: Surface levels of classical MHC class I molecules were examined as a function of time following HPPH-PDT at 0.8 J/cm2.