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Enzyme-linked immunosorbent assay (ELISA). Although most commonly used for measuring antitumor necrosis factor α (TNF) levels and antidrug antibodies, ELISA has methodologic limitations that include risk of false-positive results because of nonspecific binding to immunoglobulins, and serum levels of the respective anti-TNF may interfere with the assay and produce inconclusive results potentially leading to underestimation of antidrug antibody levels.
